單細(xì)胞轉(zhuǎn)錄組測序(Single-cell RNA-sequencing, scRNA-seq)是在單個細(xì)胞水平對mRNA進(jìn)行高通量測序的一項新技術(shù)，原理是將分離的單個細(xì)胞中微量的mRNA通過高效擴(kuò)增后再進(jìn)行高通量測序。

單細(xì)胞測序主要涉及單細(xì)胞基因組測序和轉(zhuǎn)錄組測序兩個方面，分別針對單細(xì)胞DNA及RNA進(jìn)行序列分析和比較。單細(xì)胞測序一直是科學(xué)家關(guān)注的一個熱點。

Cells, the basic units of biological structure and function, vary broadly in type and state. In most biological systems, our knowledge of cellular diversity is incomplete, as for complex tissues like the nervous system (brain cells) [1].The

想象一下有人遞給你一杯奶昔，要你鑒別出里面所有的東西?；蛟S你能夠辨別出有一點點的草莓或是酸奶的味道。

Drop-seqDroplet Based scRNA-Seq Methods